Structural and transcriptional features of the mouse spermatid genome. Isolation and characterization of an extremely basic protein from adenovirus type 5. The properties of three baculoviruses from closely related hosts. La protéine d'inclusion et les protéines du virion du baculovirus du diptére Tipula paludosa (Meigen) C R Acad Sci Hebd Seances Acad Sci D. Folding of the DNA double helix in chromatin-like structures from simian virus 40. Germond JE, Hirt B, Oudet P, Gross-Bellark M, Chambon P.Chromatin-like structures obtained after alkaline disruption of bovine and human papillomaviruses. Favre M, Breitburd F, Croissant O, Orth G.Characterization of components released by alkali disruption of simian virus 40. Christiansen G, Landers T, Griffith J, Berg P.Biophysical properties of the structural components of a granulosis virus isolated from the cabbage white butterfly (Pieris brassicae). Dissociation of polyoma virus by the chelation of calcium ions found associated with purified virions. Specific folding and contraction of DNA by histones H3 and H4. Comparative ultrastructural studies of insect granulosis and nuclear polyhedrosis viruses. Links to PubMed are also available for Selected References. Get a printable copy (PDF file) of the complete article (2.2M), or click on a page image below to browse page by page. Full textįull text is available as a scanned copy of the original print version. Electron microscopic observations suggested that the basic protein was associated with the viral DNA in the form of a DNA-protein complex. VP12 was not present in polypeptide profiles of granulosis virus capsids, indicating that it was an internal or core protein of the nucleocapsids. The basic polypeptide appeared to be virus specific because it was found in nucleocapsids and virus-infected cells but not in uninfected cells. Proteins of similar arginine content also were extracted from the granulosis virus of Pieris rapae and from the nuclear polyhedrosis viruses of Spodoptera frugiperda and Autographa californica. The remaining residues consisted primarily of serine, valine, and isoleucine. Amino acid analysis showed that 39% of the amino acid residues of VP12 were basic: 27% were arginine and 12% were histidine. Its electrophoretic mobility on acetic acid-urea gels was intermediate to that of cellular histones and protamine. ![]() One of these, a major component with a molecular weight of 12,500 (VP12), was selectively extracted from the nucleocapsids with 0.25 M sulfuric acid. When analyzed on sodium dodecyl sulfate-polyacrylamide gels, the nucleocapsids consisted of eight polypeptides. The symbol gets noted by Twitter and appears in the search or even shows up in Trending Topics if you’re lucky.Nucleocapsids were isolated from purified enveloped nucleocapsids of Plodia interpunctella granulosis virus by treatment with Nonidet P-40. Hashtags are used this way extensively by individuals and companies vying for attention on the network. You can use the hashtag anywhere in a tweet, like at the beginning, middle, or end. Adding the ‘#’ symbol before a word will enable other users to search for it and follow or retweet. Regardless of the backstory, hashtags are now a signature feature of the network, and you can do quite a lot with them.Ī hashtag is used before a keyword or phrase to make the tweet more searchable. Undeterred, Chris took his idea to the people, and hashtags were adopted by the Twitter user community first, only later earning an at-first grudging acceptance from the company. A Silicon Valley designer named Chris Messina proposed using hashtags on the new Twitter service but got shot down by the creators, who said it was “too nerdy.” Hashtags originated on IRC back in the 20th century, as users of the IRC chat applications wanted a way to categorize items into groups. Used to connect with others, various causes, and to make statements, hashtags can help you reach followers and like-minded social media users. ![]() Hashtags are so much a part of our lives now that they are finding their way into our speech patterns as well as screens.
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